Human LETM1 ELISA Kit (HUFI07716)

LETM1 (Leucine zipper-EF-hand containing transmembrane protein 1) is a mitochondrial inner membrane protein critical for ion homeostasis, particularly mitochondrial K+/H+ exchange, and is implicated in Wolf-Hirschhorn syndrome plus several cancer pathways.

This quantitative immunoassay from Assay Genie enables accurate measurement of human LETM1 protein levels across blood-derived specimens and cultured cell lysates, supporting investigations in mitochondrial biology, neurodevelopmental disorders, and tumor metabolism.

Each kit contains a pre-coated microplate, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, wash buffer, and substrate solution sufficient for a full plate run. The protocol uses 100 µL of sample per well with an overnight capture incubation at 4 °C, followed by detection steps totaling approximately 4.5 hours.

Store all unopened reagents refrigerated and protect the TMB substrate from light to preserve enzymatic activity across the kit's 12-month shelf life.