Human Leptin (LEP) ELISA Kit

A colorimetric sandwich ELISA kit from Assay Genie, designed for the quantitative measurement of leptin in human samples and supporting research into metabolism, obesity, and related health conditions. The kit recognizes natural and recombinant human leptin (synonyms: obese protein, obesity factor, OB, OBS) and is reactive with human serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids.

Reported performance characteristics include a detection range of 0.625-40ng/mL and a sensitivity of 0.26ng/mL. Intra-assay CV is 6.2% and inter-assay CV is 9.5%. Linearity has been evaluated across serum, EDTA plasma, and heparin plasma at serial dilutions, with recovery reported in the range of 92-104% for serum and 94-106% for plasma.

Leptin is described as a key regulator of energy balance and body weight, acting centrally in the hypothalamus to influence appetite and peripherally to modulate basal metabolism, reproductive function, pancreatic beta-cell activity, angiogenesis, and both innate and adaptive immunity. The associated UniProt accession is P41159, and the product is for research use only.