Human LEO1 ELISA Kit (HUFI03639)

The Human LEO1 ELISA Kit from Assay Genie quantifies LEO1, a core subunit of the PAF1 transcriptional regulatory complex that associates with RNA polymerase II to modulate chromatin remodeling, histone modification, and transcription elongation. Aberrant PAF1 activity has been implicated in Wnt signaling, stem cell maintenance, and oncogenesis across multiple cancer types, making LEO1 a relevant biomarker for studies of transcriptional dysregulation and tumor biology.

This research-use-only immunoassay employs a human-specific capture antibody on a microplate, supplied with calibrated standards, biotinylated detection reagent, streptavidin-HRP, wash buffer, and substrate. The complete workflow supports quantification of circulating LEO1 levels with a recommended sample volume of 100 µL per well. Store unopened components at 4°C, protect from light, and limit freeze-thaw cycles of working antibody solutions to preserve reagent activity through the stated shelf life.