Human LEFTY2 ELISA Kit (HUFI03635)

LEFTY2 biology: The Assay Genie ELISA measures LEFTY2 (Left-Right Determination Factor 2), a secreted TGF-β superfamily ligand that antagonizes Nodal signaling during embryonic left-right axis patterning. The kit supports investigation of stem cell pluripotency maintenance, endometrial receptivity research, and tumor suppressor pathways where LEFTY2 silencing has been implicated in carcinoma progression.

Assay workflow: The procedure relies on a human-specific capture antibody pre-coated onto a microplate, with bound analyte revealed through a chromogenic substrate reaction. Researchers load approximately 50 µL of sample per well, and reconstituted standards are aliquoted to limit freeze-thaw degradation. The unopened kit is stored refrigerated until first use, and any leftover working solution should be frozen at -20°C or below.