Human Left/Right Determination Factor 1 (LEFTY1) ELISA Kit

The Human LEFTY1 ELISA Kit from Assay Genie enables quantitative measurement of Left-Right Determination Factor 1, a secreted signaling protein of the TGF-beta superfamily implicated in embryonic left-right axis patterning, asymmetric organ morphogenesis, and pluripotency regulation in stem cell populations.

Built around a human-specific capture antibody pair, the assay delivers reproducible quantitation across tissue homogenates, cell lysates, and conditioned media samples. Researchers working in developmental biology, reproductive medicine, and oncology leverage LEFTY1 expression as a biomarker for differentiation status and tumor suppressor activity.

The microplate format accommodates 96 assays per run, with biotinylated detection antibody and streptavidin-HRP readout. Reagents should be stored at -20 degrees C in aliquots to preserve activity through multiple freeze-thaw cycles, while the working standard curve must be freshly diluted before each run.

Each kit includes a pre-coated microplate, lyophilized standards, detection antibody, HRP conjugate, substrate, stop solution, wash buffer concentrate, and adhesive plate seals. For research use only; not validated for diagnostic or clinical applications.