Human Lecithin Cholesterol Acyltransferase (LCAT) ELISA Kit

Reverse cholesterol transport research and cardiovascular risk studies benefit from a quantitative immunoassay for Lecithin Cholesterol Acyltransferase (LCAT), the enzyme that catalyses the formation of cholesteryl esters within high-density lipoprotein particles and drives the maturation of nascent discoidal HDL into spherical HDL2 and HDL3 species.

Circulating LCAT concentration is a critical indicator of HDL remodelling activity in vivo and is informative in the investigation of inherited lipid disorders such as familial LCAT deficiency and fish-eye disease.

Manufactured by Assay Genie, this microplate-format immunoassay yields 96 individual determinations per kit and is validated for use with human blood-derived specimens collected under approved study protocols.

The kit is supplied as a complete reagent set for laboratory research workflows in academic, clinical research, and pharmaceutical discovery settings, and is intended for research use only rather than diagnostic application.

End-point absorbance readout is performed on a standard plate reader, with the procedure accommodating a small aliquot of prepared specimen per well to generate a standard curve from which unknown LCAT concentrations are interpolated.

Refrigerated storage of the kit components between uses is required to maintain antibody binding activity across the stated shelf life of the product.

Assay performance has been characterised for the human target protein, supporting reliable quantification in study cohorts drawn from clinical research populations.