Human LDHA/L-lactate dehydrogenase A chain ELISA Kit

Assay Genie's double antibody sandwich ELISA kit is designed for the quantitative measurement of human L-lactate dehydrogenase A chain (LDHA) in research samples. LDHA is a key enzyme involved in the conversion of pyruvate to lactate, playing an important role in cell metabolism and energy production. The kit is intended for studies exploring LDHA dysregulation, which has been linked to diseases including cancer.

The assay has a sensitivity of 0.469 ng/mL and a detection range of 0.781-50 ng/mL. It is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported recovery ranges are 87-105% for serum (average 94%), 93-103% for EDTA plasma (average 100%), and 85-101% for heparin plasma (average 92%). Linearity across 1:2, 1:4, and 1:8 dilutions spans 89-98%, 85-104%, and 90-101% for serum; 82-94%, 82-101%, and 83-97% for EDTA plasma; and 80-94%, 81-96%, and 95-99% for heparin plasma.

The kit includes a pre-coated dismountable ELISA microplate, lyophilized standard, biotin-labeled detection antibody concentrate, HRP-streptavidin conjugate (SABC), TMB substrate, dilution buffers, stop solution, wash buffer concentrate, and a plate sealer. The kit should be stored at 2-8°C for 12 months. The associated Uniprot accession is P00338 and the research area is Cell Biology.