Human LBP (Lipopolysaccharide Binding Protein) CLIA Kit (HUES01191)

Assay Genie supplies a chemiluminescence-based immunoassay for the quantitative measurement of human Lipopolysaccharide Binding Protein (LBP) in serum, plasma, cell culture supernatants, and other biological fluids. The kit applies a Sandwich-CLIA principle on a microplate pre-coated with an antibody specific to human LBP; bound analyte is detected with a biotinylated antibody and an Avidin-HRP conjugate, and the resulting Relative Light Unit (RLU) value is read on a chemiluminescence analyser, with sample concentration determined by comparison against a standard curve.

Quoted performance figures include a sensitivity of 0.38 ng/mL, a detection range of 0.63-40 ng/mL, a recommended sample volume of 100 μL, and a total assay time of 3.5 h. The kit is reported to show no significant cross-reactivity or interference between human LBP and analogues, with both intra-assay and inter-assay coefficients of variation below 15%.

Manufacturer validation data describe spike-and-recovery ranges of 99-113% in serum, 97-110% in EDTA plasma, and 92-104% in cell culture media (n=5), with corresponding average recoveries of 106%, 104%, and 98%. Linearity has been evaluated across 1:2 to 1:16 dilutions in the same matrices. LBP is described as a key mediator in the immune response to bacterial lipopolysaccharides and a biomarker relevant to inflammatory conditions, sepsis, and other infectious diseases.