Human LAMB1/Laminin subunit beta-1 ELISA Kit

A sandwich ELISA kit from Assay Genie is designed for the quantitative measurement of human LAMB1 (Laminin subunit beta-1), a laminin subunit involved in cell adhesion and basement membrane formation. The double-antibody format uses a pre-coated capture antibody, a biotin-conjugated detection antibody, and HRP-Streptavidin with TMB substrate for colorimetric readout measured at 450 nm. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Reported sensitivity is 0.188 ng/mL with an assay range of 0.313-20 ng/mL. The kit targets Uniprot P07942 and is listed under aliases including CLM, Laminin-6 subunit beta, Laminin-2 subunit beta, Laminin-12 subunit beta, Laminin-10 subunit beta, Laminin-8 subunit beta, Laminin B1 chain, and Laminin-1 subunit beta. It is supplied for research use within the cell biology field.

Reported linearity (n=5) covers serum 89-105% (1:2), 85-99% (1:4) and 93-98% (1:8); EDTA plasma 90-104%, 91-101% and 91-99%; and heparin plasma 90-105%, 87-101% and 91-97%. Spiking recovery (n=5) averages 92% for serum, 92% for EDTA plasma and 94% for heparin plasma. Kit components are stored at 2-8°C for 12 months, with biotin-labelled antibody and HRP-Streptavidin conjugate kept away from direct light.