Human LAB7-2/CD86 (B-Lymphocyte Activation Antigen B7-2) CLIA Kit (HUES00924)

Assay Genie's CLIA platform leverages acridinium ester chemistry to generate light output proportional to CD86 concentration, typically reaching detection thresholds in the low picogram per milliliter range across a standard curve spanning roughly 7.8-500 pg/ml. The 96-well microplate format pairs a biotinylated detection antibody with streptavidin-HRP and a luminol-based substrate, and the full workflow usually completes within 3.5 hours at room temperature after an overnight capture incubation at 2-8 °C. Each plate ships in sealed foil pouches containing desiccated antibody-coated strips, eight lyophilized standards in single-use vials, and a 25x concentrated wash buffer that requires dilution in ultrapure water before use.

Investigators profiling tumor-infiltrating lymphocytes, monocyte-derived dendritic cells, and B-cell lymphoma lines commonly pair CD86 quantitation with flow cytometry to confirm surface expression patterns. The protocol tolerates up to 200 µl of diluted serum per well, and EDTA plasma is recommended over heparinized plasma to minimize coagulation-related background interference. Reconstituted detection antibody remains stable for 7 days at 2-8 °C when stored protected from light, while the chemiluminescent substrate should be equilibrated to ambient temperature before dispensing to maximize signal uniformity across wells.