Human L1CAM/CD171 (L1-Cell Adhesion Molecule) CLIA Kit (HUES01044)

For reliable performance, bring all kit components to room temperature (18-25 °C) for at least 30 minutes before use, and reconstitute any lyophilized standards with the supplied dilution buffer immediately prior to running the plate. A typical standard curve is generated from a seven-point serial dilution spanning roughly 7.8 to 500 pg/mL, with samples and calibrators plated in duplicate so that intra-assay coefficients of variation stay below 10%. The capture antibody is pre-immobilized on a 96-well microplate, the detection antibody is conjugated to horseradish peroxidase, and signal is developed with a luminol-based chemiluminescent substrate that is read on a glow-type luminometer at an integration time of 0.1-1 second per well. Sample concentrations are then interpolated against the standard curve using a four-parameter logistic fit, which handles the wide dynamic range without requiring repeat dilutions of most clinical samples.

Researchers commonly apply this Assay Genie CLIA kit to quantify circulating L1CAM in serum or plasma from oncology patients, where elevated concentrations have been reported in pancreatic ductal adenocarcinoma, gastric carcinoma, colorectal cancer, and high-grade gliomas. Additional applications include measuring soluble L1CAM shedding into cerebrospinal fluid in neuroscience studies of axon regeneration and synaptic plasticity, profiling conditioned media from neuronal or glial cell culture models, and validating L1CAM/CD171 expression in tumor xenograft or organoid workflows. The 96-well format readily accommodates side-by-side comparison of patient cohorts, dose-response treatments, or time-course experiments within a single run.

Unopened components should be stored at 2-8 °C and remain stable until the printed expiration date, while reconstituted standards are best aliquoted and held at -20 °C or lower to limit freeze-thaw cycles. The luminescent substrate must be protected from light, and a fresh standard curve is recommended with each new plate. This research-use-only in vitro kit is compatible with both manual pipetting workflows and automated liquid-handling systems, and Assay Genie provides technical guidance on protocol optimization and data interpretation.