Human Kruppel Like Factor 15 (KLF15) ELISA Kit

KLF15 (Kruppel-like factor 15) is a zinc-finger transcription factor that regulates gluconeogenesis, lipid metabolism, and cellular responses to glucocorticoid and fasting signals across metabolically active tissues including liver, kidney, heart, and skeletal muscle. The Assay Genie ELISA kit provides a quantitative read-out of endogenous human KLF15 protein, intended for research use only, supporting investigations into diabetes, cardiac hypertrophy, and renal physiology.

Each kit is configured as 96 assays with a colorimetric end-point. The workflow uses a pre-coated plate, biotinylated detection antibody, and streptavidin-HRP conjugate chemistry; absorbance values are interpolated against a provided standard curve. Validated sample types include tissue homogenates and cell lysates, allowing measurement of KLF15 from cultured cells or dissected tissue.

Reagents are shipped refrigerated and should be stored according to the package insert until first use to preserve antibody binding activity. Researchers studying metabolic gene regulation, stress signalling, or tissue-specific transcription factor activity can integrate this assay alongside western blot, qPCR, or chromatin immunoprecipitation workflows to cross-validate transcriptional readouts with protein abundance.