Human Krueppel-like factor 10 (KLF10) ELISA Kit

A high-quality Sandwich ELISA Kit from Assay Genie designed for the quantitative measurement of human Krueppel-like factor 10 (KLF10) in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. KLF10, also known as TGF-beta-inducible early gene 1 (TIEG1), is a transcription factor that plays a crucial role in regulating gene expression, cell differentiation, cell proliferation, apoptosis, and immune response. Dysregulation of KLF10 has been linked to diseases such as cancer, fibrosis, and inflammatory disorders, highlighting its significance as a potential therapeutic target.

The kit uses a colorimetric, sandwich-based detection method with specificity for natural and recombinant human KLF10. Performance characteristics include a detection range of 0.156–10 ng/mL and a sensitivity of 0.1 ng/mL. Intra-assay CV is 5.2% and inter-assay CV is 8.5%. Linearity has been validated in serum (106–116%, 113–123%, 107–117%, 81–90%), EDTA plasma (110–118%, 94–105%, 99–109%, 101–110%), and heparin plasma (103–116%, 110–120%, 82–92%, 98–106%) across dilutions from 1:2 to 1:16. Recovery in serum averages 98% (range 92–104%) and in plasma averages 100% (range 94–106%).

Synonyms for the target include EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1, TGFB-inducible early growth response protein 1, TIEG, and TIEG1 (UniProt: Q13118). The protein localises to the nucleus and the kit is intended for research use only, with a research area focused on cancer.