Human KRT81 ELISA Kit (HUFI03656)

Keratin 81 (KRT81) is a type II hair keratin expressed in the cortex of the hair follicle, where it forms intermediate filaments that contribute to hair shaft strength. Loss-of-function variants in the KRT81 gene on chromosome 12q13.13 are linked to monilethrix, an inherited disorder characterised by beaded hair and fragility, making the protein a target of interest in dermatology and trichology research. The Assay Genie Human KRT81 ELISA Kit provides a quantitative immunoassay for measuring KRT81 levels in human samples, supporting studies of hair follicle biology, alopecia mechanisms, and keratinocyte differentiation.

Each kit is supplied in a 96 Assays format containing pre-coated microplate wells, detection reagents, and a standard curve sufficient to quantify recombinant and native KRT81 in validated sample matrices. The assay uses a colorimetric readout measurable on a standard plate reader, with reagents prepared according to the enclosed protocol and stored refrigerated between uses. Reconstituted standards should be aliquoted and kept frozen to limit freeze-thaw cycles, and the full insert provides guidance on sample dilution, incubation times, and calculation of results.

Assay Genie provides lot-specific quality control data and technical documentation with every shipment. The kit supports research workflows in hair disorder screening, where quantifying residual KRT81 protein expression can inform genotype-phenotype correlations in monilethrix families. Researchers studying hair biology, epithelial differentiation, or keratin-based biomarkers will find this kit a focused tool for KRT81 quantification.