Human KLHL9 ELISA Kit (HUFI03666)

The Human KLHL9 ELISA Kit from Assay Genie provides quantitative measurement of Kelch-like protein 9 (KLHL9), a BTB-domain substrate adaptor that operates within CUL3-RING E3 ubiquitin ligase complexes. The immunoassay enables reproducible detection of human KLHL9 across blood-derived biospecimens.

Capture antibodies are human-specific, with no significant cross-reactivity against related BTB-Kelch family proteins. Researchers investigating ubiquitination pathways, mitotic regulation, and BTB-domain adaptor biology can apply this kit to biomarker profiling studies, including research into cancer cell proliferation and protein degradation mechanisms.

Each kit contains a pre-coated microplate, biotinylated detection antibody, recombinant KLHL9 standard, streptavidin-HRP conjugate, wash buffer concentrate, TMB substrate, and stop solution. Standard protocols require a 100 uL sample volume per well, with a total incubation time of approximately 3.5 hours. Unopened components should be refrigerated and protected from light; reconstituted standards and diluted detection antibody should be aliquoted and frozen for longer-term stability.

This product supports research into the role of KLHL9 in CUL3-dependent proteasomal targeting, including its established interactions with KLHL13 and BTB domain-mediated dimerization during mitotic regulation.