Human KLHL35 ELISA Kit (HUFI03684)

Quantitative immunoassay for measuring human Kelch-like protein 35 (KLHL35) in research sample matrices, manufactured by Assay Genie as a complete research kit. The assay uses a colorimetric readout on a microplate format and is configured for 96 Assays, providing enough reagents for a full plate of unknown samples, standards, and controls.

KLHL35 belongs to the BTB-Kelch protein family, members of which participate in substrate adaptor roles for Cullin-3-based E3 ubiquitin ligase complexes. Detecting circulating levels of this protein is relevant to research programs investigating muscle biology, developmental signaling pathways, and exploratory biomarker discovery in human cohorts. The kit is supplied as a ready-to-run set of pre-coated wells, detection antibody, standards, and buffer solutions for use in qualified laboratory settings.

Each kit is intended for research use only and should be handled according to standard laboratory practices for protein-based assays. Storage conditions and reagent preparation steps are detailed in the supplied manual; users are encouraged to equilibrate components to room temperature before initiating the protocol and to avoid repeated freeze-thaw cycles of working aliquots. With Assay Genie's documentation and batch-specific calibration, laboratories can integrate KLHL35 quantification into broader protein profiling workflows alongside other ELISA-based readouts.