Human KLHL13 ELISA Kit (HUFI03686)

Quantitative measurement of human Kelch-like 13 (KLHL13) protein is enabled by this in vitro ELISA kit from Assay Genie. KLHL13 is a BTB-domain substrate adaptor that recruits target proteins to CUL3-based E3 ubiquitin ligase complexes, driving ubiquitin-dependent proteolysis. The kit contains pre-coated microplate wells, biotinylated detection antibody, streptavidin-HRP conjugate, wash concentrate, TMB substrate, stop solution, and recombinant KLHL13 calibrators sufficient for 96 Assays. Researchers studying Cullin-RING ligase signaling, neuromuscular junction biology, and ubiquitin-proteasome pathway regulation use this assay to profile relative KLHL13 abundance across experimental cohorts. Each well is processed through an add-sample-incubate-wash-develop protocol and read on a conventional microplate spectrophotometer; absorbance values are back-calculated against the calibrator curve. Reagents are supplied in ready-to-use vials and tubes; store at refrigerated temperature upon arrival and equilibrate components to room temperature before each run. Intended for research use only.