Human Kinesin Family, Member 5B (KIF5B) ELISA Kit

KIF5B encodes the heavy chain of kinesin-1, a microtubule-based motor protein essential for axonal transport, mitochondrial positioning, and vesicle trafficking in neurons. Researchers investigating this target frequently examine its role in neurodegenerative disease models, intracellular cargo transport mechanisms, and oncology applications—particularly the well-characterized KIF5B-RET fusion transcripts documented in non-small cell lung adenocarcinoma.

This ELISA Kit from Assay Genie is supplied in a 96-assay microplate format with absorbance-based readout and uses a human-specific capture antibody, supporting quantitative measurement of human KIF5B protein in tissue homogenate preparations. The protocol follows a research-use-only workflow; kit components require refrigerated storage between runs, and reconstituted standards or detection antibody should be aliquoted to limit repeated freeze-thaw cycles. Standard curve generation, recommended dilution buffers, and troubleshooting guidance are included in the supplied insert, enabling reproducible interassay comparison across tumor tissue, primary culture, and cell lysate samples.