Human KIF5B ELISA Kit (HUFI03713)

The Human KIF5B ELISA Kit from Assay Genie provides quantitative measurement of Kinesin Family Member 5B, the heavy chain of conventional kinesin-1 that drives microtubule-dependent anterograde transport of organelles, mRNA granules, and receptor-bearing vesicles in human cells.

KIF5B has emerged as a research focus in lung adenocarcinoma biology, where KIF5B-RET gene fusions produce chimeric oncoproteins, and in neuroscience studies of axonal cargo trafficking, mitochondrial positioning, and mitotic spindle assembly.

Supplied as a complete assay package from Assay Genie, the kit includes a pre-coated microplate, lyophilized recombinant standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer concentrate, and chromogenic substrate. Researchers typically load 100 uL of sample per well and read absorbance with a microplate reader after the enzymatic reaction, with standard curves constructed from serial dilutions to determine unknown KIF5B concentrations in research specimens.