Human KIF5A/Kinesin 5A ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA kit employs a double-antibody format for the quantitative detection of human Kinesin-5A (KIF5A) levels in research samples. The colorimetric assay uses a TMB substrate with absorbance read at 450 nm. KIF5A is described as a motor protein involved in intracellular transport, particularly the movement of organelles and vesicles along microtubules, and its dysregulation has been linked to neurodegenerative disorders, making it a biomarker of interest for related research.

Reactivity is human-specific. The kit reports a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Validated sample types include serum, plasma, cell or tissue lysate, and other liquid samples, with storage at 2-8°C for 12 months. Linearity in serum (n=5) ranges from 92-105% at 1:2, 86-94% at 1:4, and 80-99% at 1:8 dilution; EDTA plasma (n=5) shows 87-93%, 88-97%, and 81-92%; heparin plasma (n=5) shows 85-95%, 83-101%, and 82-100%. Recovery ranges are 89-104% (average 97%) for serum, 93-105% (average 100%) for EDTA plasma, and 88-96% (average 91%) for heparin plasma.

The procedure involves primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding for 60 minutes, HRP-streptavidin incubation for 30 minutes, and TMB color development for 10-20 minutes in the dark, followed by stop solution and immediate absorbance measurement at 450 nm.