Human KIAA0513 ELISA Kit (HUFI03732)

The Assay Genie Human KIAA0513 ELISA Kit is a quantitative immunoassay for measuring KIAA0513 protein levels in human research samples. KIAA0513 is a protein-coding gene investigated in neuronal and developmental biology contexts, and the kit supports studies exploring its expression across human-derived specimens. The microplate is pre-coated with a capture antibody specific to human KIAA0513, and detection proceeds through a biotin-streptavidin-HRP readout with TMB substrate. The protocol typically requires 100 µL of prepared sample per well.

Reagents supplied include a pre-coated plate, biotinylated detection antibody, lyophilized standard reference, wash concentrate, dilution buffer, streptavidin-HRP conjugate, substrate, and stop solution. The plate layout supports an eight-well standard curve alongside samples run in duplicate or triplicate. Researchers should aliquot reagents to limit freeze-thaw cycles and refer to the lot-specific certificate of analysis for exact working concentrations and recommended dilution factors.