Human KIAA0101/PCNA-associated factor ELISA Kit

Developed by Assay Genie, this sandwich ELISA kit is intended for the quantitative measurement of KIAA0101/PCNA-associated factor in human samples. The assay uses a colorimetric detection method in a double-antibody sandwich format and has been validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. KIAA0101/PCNA-associated factor interacts with proliferating cell nuclear antigen (PCNA) and has a critical role in cell cycle progression and DNA replication; its dysregulation has been implicated in cancer, making it a useful biomarker for studying disease mechanisms and therapeutic targets.

The kit has a sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL. Linearity in serum (n = 5) is 86–104% at 1:2, 92–101% at 1:4, and 81–93% at 1:8; in EDTA plasma (n = 5) 93–100%, 84–101%, and 81–95%; and in heparin plasma (n = 5) 90–103%, 88–101%, and 81–96%. Average recovery is 94% in serum (range 87–99%), 100% in EDTA plasma (range 94–104%), and 95% in heparin plasma (range 86–100%). Kits should be stored at 2–8°C for 12 months, with the TMB substrate, biotin-labeled antibody, and HRP-streptavidin conjugate protected from direct light.

The protocol includes reagent equilibration, a 90-minute primary incubation at 37°C, biotinylated detection antibody incubation for 60 minutes at 37°C, HRP-streptavidin (SABC) incubation for 30 minutes at 37°C, TMB color development for 10–20 minutes in the dark, and absorbance measurement at 450 nm after the stop reaction. Sample preparation guidance covers serum (centrifugation at 1000 × g for 20 minutes), plasma (centrifugation at 1000 × g for 15 minutes at 2–8°C), cell culture supernatant, cell lysate, and tissue homogenate workflows.