Human KIAA0087 ELISA Kit (HUFI03727)

The Assay Genie Human KIAA0087 ELISA Kit enables quantitative measurement of KIAA0087 protein in human serum samples and plasma specimens using a matched antibody pair configuration read on a standard microplate reader. Each kit provides a pre-coated 96-assay plate, biotinylated detection antibody, streptavidin-HRP conjugate, protein standards, wash buffer, substrate solution, and stop reagent sufficient for one full plate run.

Researchers studying gene products originally catalogued in the Kazusa cDNA project can use this kit to investigate expression patterns across disease cohorts and matched controls. The colorimetric readout produces a standard curve from which unknown concentrations are interpolated, with results reported in ng/mL relative to the supplied calibrators. The protocol requires 100 µL of diluted sample per well and uses a small number of pipetting steps compatible with routine laboratory workflows.

For best stability, store unopened kit components at refrigerated temperatures and avoid repeated freeze-thaw cycles of the reconstituted standards. The full assay can be completed in approximately 4 hours, and the plate layout supports both full-plate runs and smaller batch analyses using partial strip configurations. Researchers are advised to validate diluent compatibility and matrix effects for their specific clinical or experimental cohorts before drawing quantitative conclusions.