Human Keratinocyte proline-rich protein/KPRP ELISA Kit

For researchers studying keratinocyte biology and epidermal differentiation, the Human Keratinocyte proline-rich protein (KPRP) ELISA Kit from Assay Genie offers quantitative measurement of KPRP in human-derived research specimens. KPRP is a structural proline-rich protein expressed during late-stage keratinocyte cornification, contributing to cornified envelope formation in stratified squamous epithelia. Applications include dermatological investigations of skin barrier integrity, psoriasis vulgaris, atopic dermatitis, and cutaneous wound healing models.

The kit is supplied as a complete reagent set organized around a microplate format capable of running 96 determinations per plate. Each well uses a matched antibody pair format, with KPRP captured between an immobilized antibody on the plate and a conjugated detection antibody. After a wash and substrate step, absorbance is read on a standard microplate reader. Reconstituted standards and detection antibody should be stored refrigerated and protected from light; the working protocol typically runs two to three hours from loading to final readout.

Investigators examining stratum corneum composition, epithelial barrier defects, or inflammatory skin conditions can apply this assay to cell culture supernatants, tissue homogenates, and human serum or plasma-derived specimens. The product is intended for research use only and is not validated for clinical diagnostic use.