Human Keratin, type I cytoskeletal 18 (KRT18) ELISA Kit

Assay Genie provides this Sandwich ELISA Kit for the quantitative measurement of human Keratin, type I cytoskeletal 18 (KRT18) in research samples. KRT18 is a type I cytoskeletal keratin that contributes to the structural integrity of epithelial cells, and abnormal levels of the protein have been associated with liver disease, cancer, and inflammatory disorders, making it a useful biomarker for cell biology and disease pathology research.

The kit is intended for use with human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. It employs a Sandwich ELISA format with colorimetric detection, and the antibody pair is specific for natural and recombinant human Keratin, type I cytoskeletal 18. Reported synonyms for the target include Cytokeratin-18, Keratin-18, CK-18, K18, CYK18, PIG46, and Cell proliferation-inducing gene 46 protein (UniProt P05783).

Performance characteristics supplied by Assay Genie include a detection range of 62.5-4000pg/mL and a sensitivity of 19.5pg/mL, with intra-assay CV of 4.6% and inter-assay CV of 8.0%. Spike-and-recovery in serum averaged 97% (range 91-103%) and in plasma 99% (range 93-105%), while linearity was demonstrated across 1:2 to 1:16 dilutions in serum, EDTA plasma and heparin plasma. The kit is supplied for research use only.