Human KCNJ1 ELISA Kit (HUFI03743)

Researchers investigating renal potassium channel biology can use the Assay Genie Human KCNJ1 ELISA Kit to quantify KCNJ1 protein levels, also known as ROMK (renal outer medullary potassium channel), in human-derived specimens. The kit is intended for research use only and supports studies examining KCNJ1 expression in nephron segments, ion transport physiology, and the molecular basis of Bartter syndrome type II, where loss-of-function mutations in KCNJ1 impair potassium recycling in the thick ascending limb of the loop of Henle.

Each kit supplies reagents sufficient for 96 assays, including pre-coated plate wells, detection antibody, lyophilized standards, and assay buffer components needed to construct a calibration curve and run unknown samples alongside. The workflow supports parallel processing of standards and unknowns on a single microplate.

Applications include renal channelopathy research, hypertension mechanism studies, and electrolyte balance disorder investigations. By providing reproducible quantification, the kit enables comparative analyses between healthy donor cohorts and patient populations, complementing genetic sequencing and electrophysiological patch-clamp approaches to channel function. Reagents should be refrigerated between uses to preserve antibody binding activity and standard integrity.