Human Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) ELISA Kit

This Assay Genie sandwich ELISA enables quantitative measurement of KAZALD1, also known as BONO1, a secreted Kazal-domain serine protease inhibitor implicated in osteoblast differentiation, bone formation, and IGF signaling. Researchers studying altered KAZALD1 expression in breast carcinoma, glioma models, and skeletal tissue development can apply this assay to tissue homogenates or cell lysates using a 100 uL sample volume per well. The human-specific capture antibody shows no significant cross-reactivity with mouse or rat homologs, supporting clean discrimination in studies of IGFBP-related signaling, cell adhesion, and extracellular matrix remodeling. Reagents are supplied in a 96-well microplate with pre-coated plate, standards, detection antibody, and substrate buffer; store unopened components refrigerated at 2-8°C, protect from light, avoid freeze-thaw cycles of working solutions, and read absorbance at 450 nm on a standard plate reader.