Human Kallikrein 6 protein ELISA Kit

Designed for the precise measurement of Kallikrein 6 levels in human samples, this Sandwich ELISA Kit from Assay Genie offers high sensitivity and specificity for a variety of research applications. The kit is suitable for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. It operates across a detection range of 0.313–20 ng/mL with a sensitivity of 0.188 ng/mL, using a colorimetric, double-antibody sandwich detection method.

Kallikrein 6 is a key protein involved in tissue remodeling, inflammation, and neural plasticity. Its dysregulation has been linked to conditions such as cancer, Alzheimer's disease, and multiple sclerosis, making it a valuable biomarker for investigating disease pathology and potential therapeutic interventions. The assay follows a defined protocol: reagents and TMB substrate are equilibrated to room temperature, samples and standards are incubated at 37°C for antigen binding, a biotin-labeled detection antibody and HRP-Streptavidin conjugate are applied sequentially, and color development with TMB substrate is read at 450 nm after adding stop solution.

Supplied components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody concentrate, HRP-Streptavidin conjugate, TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer, plate sealer, and a technical manual. The kit should be stored at 2–8°C and remains stable for 12 months under these conditions.