Human JIP3 ELISA Kit (HUFI07758)

This Assay Genie kit enables researchers to quantify endogenous human JIP3 (also known as MAPK8IP3), a JNK pathway scaffold protein studied in the context of axonal transport, neuronal stress responses, and neurodegenerative disease mechanisms. The two-antibody capture format is read on a standard microplate reader with chromogenic substrate, and the assay covers a detection range of approximately 0.156–10 ng/mL with a sensitivity of around 0.08 ng/mL. Each pack ships refrigerated on wet ice; unopened components are stored cold and protected from light, with reconstituted standards stable through several freeze-thaw cycles when aliquoted. Researchers commonly apply it to clinical fluid samples from cohort studies, neuronal cell culture models, and brain tissue lysates, with cross-reactivity validated against human, mouse, and rat MAPK8IP3 isoforms. Components include a pre-coated microplate, biotinylated detection reagent, enzyme conjugate, calibrated recombinant human standards, wash buffer, and chromogenic substrate. The kit supports in vitro investigation of MAP kinase signaling, tau phosphorylation, and amyloid-beta interactions, and is used in diabetes, oncology, and neuroscience research where scaffold protein dynamics inform disease progression.