Human JAM-C PharmaGenie ELISA Kit (SBRS0806)

The Assay Genie Human JAM-C ELISA quantifies Junctional Adhesion Molecule-C, an immunoglobulin-superfamily protein involved in endothelial tight-junction integrity, leukocyte transmigration, and angiogenic signaling. This 96-test kit supports research into cardiovascular inflammation, tumor cell extravasation across vessel walls, neutrophil recruitment in inflammatory disease models, and blood-testis barrier function in reproductive biology. Altered JAM-C expression has been documented in atherosclerotic plaques, multiple sclerosis lesions, and several solid tumor microenvironments, making reliable quantification valuable for translational research programs.

The assay is configured for cell-culture supernatant, plasma samples, and serum samples, and follows a two-antibody format on a microplate. Each kit provides the pre-coated plate, capture and detection antibodies, recombinant JAM-C reference standard, and substrate reagents required to complete 96 individual reactions. Standard preparation involves serial dilution of the provided reference standard, after which samples and standards are added to antibody-coated wells. Following incubation and wash steps, a chromogenic substrate reaction produces a measurable signal proportional to JAM-C concentration, read on a standard plate reader.

Store unopened kit components refrigerated; bring reagents to room temperature before use. The assay is intended for research use only and should not be used for diagnostic procedures.