Human Isoleucyl tRNA Synthetase (IARS) ELISA Kit

Isoleucyl-tRNA Synthetase (IARS) catalyzes the ATP-dependent attachment of isoleucine to its corresponding transfer RNA, a foundational step in maintaining translational fidelity for this branched-chain amino acid. The Assay Genie kit provides a quantitative immunoassay for measuring human IARS protein levels, supporting translational research, biomarker discovery, and mechanistic studies in cell and tissue preparations.

Biallelic mutations in IARS1 are associated with a rare autosomal recessive disorder characterized by intrauterine growth retardation, microcephaly, intellectual disability, and sensorineural hearing loss, making reliable IARS quantification valuable for disease modeling and patient-derived investigations. Upregulation of IARS has additionally been reported in hepatocellular carcinoma and certain leukemias, where this assay can be used to evaluate aminoacyl-tRNA synthetase expression patterns as part of broader proteomic profiling efforts.

Working with a recommended sample volume of 50 uL per well, the microplate format accommodates standard curves, internal controls, and replicate samples in a single run. Reagents are stored refrigerated and should be protected from light until use; bring all components to room temperature before beginning the protocol. Intended for research use only, not for diagnostic applications.