Human Isocitrate Dehydrogenase 2,mitochondrial (IDH2) ELISA Kit

Mitochondrial isocitrate dehydrogenase 2 (IDH2) catalyzes a key oxidative decarboxylation step in the tricarboxylic acid cycle, and quantifying this enzyme is central to research on cancer metabolism, glioma biology, and acute myeloid leukemia, where IDH2 hotspot mutations (R140 and R172) are widely studied. The Assay Genie Human Isocitrate Dehydrogenase 2, mitochondrial (IDH2) ELISA Kit provides a quantitative in vitro immunoassay developed specifically for the human enzyme. Researchers apply the kit to tissue homogenates, cell lysates, cell culture supernatants, and other human specimen types to measure IDH2 protein levels across experimental conditions. Each kit ships with a pre-coated microplate, biotinylated detection antibody, recombinant IDH2 standards, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent. The absorbance-based read-out integrates with standard microplate readers, and the kit is intended for research use only. Store unopened reagents refrigerated and protect components from light to preserve activity through the stated shelf life.