Human Ischemia Modified Albumin (IMA) ELISA Kit

A sandwich ELISA from Assay Genie designed for the quantitative measurement of Ischemia Modified Albumin (IMA) in human samples. The kit uses a colorimetric detection method and is intended for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Reported performance characteristics include a detection range of 7.8–500 ng/mL and a sensitivity of 3.1 ng/mL, with an intra-assay CV of 4.2% and an inter-assay CV of 8.1%. Specificity is stated for natural and recombinant human Ischemia Modified Albumin, and the product is labelled for research use only.

Background supplied with the kit notes that IMA can be detected via the albumin cobalt binding (ACB) test, described as a limited available FDA approved assay. Myocardial ischemia alters the N-terminus of albumin, reducing the ability of cobalt to bind to albumin, and IMA measures ischemia in the blood vessels, returning results in minutes rather than the hours required by traditional markers of necrosis. The source text adds that the ACB test has low specificity, generating a high number of false positives, and must be used in conjunction with typical acute approaches such as ECG and physical exam, with additional studies required.