Human Iron Responsive Element Binding Protein 2 (IREB2) ELISA Kit

Built for quantitative measurement of human IREB2, this in vitro ELISA kit from Assay Genie supports research into cellular iron homeostasis and the post-transcriptional regulation governed by iron regulatory proteins. IREB2 (Iron Responsive Element Binding Protein 2), also referred to as IRP2, is a cytosolic sensor that binds iron-responsive elements in untranslated regions of mRNAs encoding proteins involved in iron uptake, storage, and export, including transferrin receptor 1 and ferritin.

The kit is supplied as a complete reagent set, including pre-coated microplate wells, detection antibody, reference standard, enzyme conjugate, wash buffer, substrate, and stop solution, sufficient for 96 assays. Investigators studying iron metabolism disorders, neurodegeneration, mitochondrial function, and pulmonary disease use this ELISA to quantify IREB2 levels across human sample matrices, including serum samples, plasma samples, tissue homogenates, and cell lysates. Each run produces a standard curve from which unknown concentrations are interpolated, and the assay is intended for research use only.

Storage recommendations are listed in the product insert; kit components are kept refrigerated until first use, with working dilutions prepared fresh before each experiment. Aliquoting and limiting freeze-thaw cycles of reconstituted standards helps preserve binding activity. The procedure follows a two-antibody capture workflow compatible with common microplate readers capable of absorbance measurement at the wavelength specified in the manual.

Assay Genie supplies the kit with a complete protocol, lot-specific certificate of analysis, and technical support for assay optimization. For investigators comparing IREB2 expression between patient cohorts, disease models, or knockdown and overexpression systems, the kit offers a reproducible plate-based readout of protein abundance that complements mRNA-level analyses such as qPCR and protein-level studies such as western blot.