Human IQUB (IQ and ubiquitin-like domain-containing protein) ELISA KIT (HUFI05127)

Beyond the core quantitative workflow, this Assay Genie kit ships with a pre-coated strip plate, lyophilized recombinant IQUB standard for curve generation, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, dilution buffers, chromogenic TMB substrate, and a stop solution. The standard curve is constructed from a serial dilution series and unknown sample concentrations are interpolated against the fitted four-parameter logistic curve. The recommended assay workflow involves adding 100 µL of standard or diluted sample per well, incubating, washing, adding 50 µL of detection reagent, and reading absorbance after substrate addition and stop solution. Researchers studying male infertility phenotypes benefit from pairing this kit with semen parameter analysis and genetic screening for IQUB variants, while those focused on motile cilia biology can use it to examine IQUB expression across different tissues or under RNAi knockdown conditions. The kit is shipped refrigerated on blue ice packs and is intended for laboratory research use only, not for diagnostic or clinical decision-making.