Human Interleukin 8 (IL-8) ELISA Kit

IL-8 (also known as CXCL8) is a pro-inflammatory chemokine that drives neutrophil recruitment and activation. Quantitative measurement of IL-8 supports research into acute inflammation, infection, sepsis, respiratory conditions, autoimmune disease, and tumor microenvironment biology. Assay Genie supplies this Human Interleukin 8 ELISA Kit to quantify endogenous IL-8 in human samples for research use only.

This 96-assay kit is validated for human serum samples, human plasma samples, tissue homogenates, cell lysates, and cell culture supernatants, giving laboratories flexibility to bridge clinical specimens with in vitro stimulation and cell-based work. The assay uses a chromogenic read-out, and all reagents are supplied as ready-to-use solutions or as concentrated stock that the user dilutes per the included protocol. For stability, the unopened kit should be kept refrigerated; reconstituted standards and working solutions should be aliquoted and frozen for longer-term storage, with repeated freeze-thaw cycles of prepared standards avoided. Bring all reagents to room temperature before beginning the assay.

Common applications include profiling cytokine release in stimulated peripheral blood mononuclear cells, measuring IL-8 in tumor cell line supernatants, screening anti-inflammatory compounds in vitro, and quantifying inflammatory mediators in patient-derived tissue lysates. The microplate-based format supports standard curve generation with recombinant IL-8 standards and parallel processing of test samples.