Human Interleukin 33 (IL-33) ELISA Kit

Assay Genie provides a quantitative immunoassay reagent set built around Interleukin-33, a member of the IL-1 cytokine family that localizes to the nucleus under homeostatic conditions and is released as an alarmin upon epithelial or endothelial damage. The full-length IL-33 precursor runs at approximately 30 kDa and is cleaved by inflammatory proteases into a shorter mature form with enhanced biological activity. IL-33 signals through the ST2 receptor (IL1RL1) to drive Th2 cytokine production, expand ILC2 populations, and activate mast cells and eosinophils, making it a frequently quantified biomarker in allergy, asthma, atopic dermatitis, rheumatoid arthritis, and pulmonary fibrosis research programs.

The reagent set is supplied as a complete microplate-based kit with all buffers, conjugate, substrate, and reference standard included. The absorbance-based readout integrates with standard plate readers commonly found in immunology and cell biology laboratories, supporting ELISA workflows for protein quantification.

Validated input matrices for the workflow span fresh and archived clinical specimens, tissue homogenates, cell lysates, and cell culture supernatant fractions, covering both translational samples and outputs from in vitro experimental systems. The kit is labeled for research use only and is not intended for diagnostic procedures. Storage recommendations accompany the package insert; working reagents should generally be kept refrigerated until first use, with standard aliquots prepared to limit freeze-thaw exposure of the reference material.