Human Interleukin 27 (IL-27) ELISA Kit

The Assay Genie Human IL-27 ELISA Kit quantifies interleukin-27, a heterodimeric cytokine composed of EBI3 and p28 subunits that regulates Th1 differentiation, cytotoxic T cell activity, and inflammatory signaling. Researchers apply this kit to measure IL-27 release in disease models spanning autoimmunity, chronic infection, and tumor immunology.

Specifications: detection range 15.6 to 1000 pg/mL, sensitivity 5 pg/mL, 100 µL sample volume per well, and a standard curve fitted with recombinant IL-27 calibrator. Each microplate is provided pre-coated with human-specific capture antibody and paired with biotinylated detection reagent, streptavidin-HRP, and TMB substrate. Reconstituted standards remain stable through multiple freeze-thaw cycles when aliquoted and stored at -20°C; the unused strip plate should be refrigerated at 4°C in its foil pouch with desiccant.

The assay supports research use only applications in cytokine profiling, immune cell stimulation studies, and biomarker discovery workflows. Researchers can complete the full plate in under 3 hours following the included protocol, then read absorbance values on a standard microplate reader.