Human Interleukin 12A (IL-12A) ELISA Kit

The Human Interleukin 12A (IL-12A) ELISA Kit from Assay Genie is built around a human-specific capture antibody pair, enabling selective quantification of the IL-12p35 subunit in cell culture supernatants and tissue lysates. IL-12A encodes the p35 chain that pairs with IL-12B (p40) to form bioactive IL-12, a key driver of Th1 differentiation and IFN-gamma production by T cells and NK cells. Researchers use this kit to study cytokine responses in infectious disease, tumor immunology, autoimmune inflammation, and vaccine adjuvant development, measuring p35 secretion to characterize cellular immune activity. The microplate arrives pre-coated and ready for a 100 µL sample volume per well, with lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and stop solution supplied in the pack. Unopened components should be stored refrigerated, protected from light, with working diluents kept on ice during the assay run.