Human Interleukin 10 (IL-10) ELISA Kit

Quantify human interleukin-10 with this quantitative immunoassay from Assay Genie, configured for two-site antibody capture with a colorimetric readout. IL-10 is a key anti-inflammatory cytokine released by Th2 lymphocytes, regulatory T cells, monocytes, and macrophages, and its measurement supports research into immune regulation, chronic inflammation, tumor microenvironment immunology, autoimmune disease models, and infectious disease studies.

The kit accepts a broad range of human sample matrices, including whole serum, heparin or EDTA plasma, tissue homogenates prepared by mechanical disruption, cell lysates from cultured or isolated populations, and clarified cell culture supernatants. Each kit provides reagents for 96 individual determinations and ships with a pre-coated microplate, lyophilized recombinant IL-10 standard, biotinylated detection antibody, streptavidin-HRP conjugate, concentrated wash buffer, TMB substrate solution, and stop reagent.

Typical workflow involves pipetting diluted standard or sample into the antibody-coated wells, incubating with the biotinylated detection reagent, washing, adding streptavidin-HRP and substrate, then reading absorbance on a standard plate reader. Standard curves are generated from serial dilutions of the provided recombinant human IL-10 reference material, and results are interpreted against the resulting calibration curve.

This product is intended for research use only and is not validated for diagnostic procedures. Storage conditions, expiration dating, and detailed lot-specific performance characteristics are documented in the certificate of analysis shipped with each kit.