Human Interferon Regulatory Factor 2 Binding Protein 2 (IRF2BP2) ELISA Kit

Protein Background

The Assay Genie IRF2BP2 ELISA Kit quantifies Interferon Regulatory Factor 2 Binding Protein 2 in human samples. IRF2BP2 functions as a transcriptional co-repressor that binds IRF-2, modulating expression of interferon-stimulated genes and influencing lymphocyte maturation. The kit employs a human-specific capture antibody to selectively measure endogenous IRF2BP2 in research applications.

Kit Contents

Each package contains a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibration standards, and assay diluent in sealed vials. Reconstituted reagents should be stored at -20 degrees C to preserve enzymatic activity, while unopened components remain shelf-stable until the printed expiration date. For research use only.

Workflow

Dispense 50 uL of prepared sample into each well of the assay plate, incubate, wash, add 100 uL of detection reagent, develop with TMB substrate, and quantify. Full plate completion requires approximately 4.5 hours, after which absorbance values are interpolated against the standard curve for downstream concentration calculations in immunology, oncology, and cell signaling studies.