Human Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) in human serum, plasma, and cell culture supernatants. The assay uses a colorimetric detection method and is intended for research applications exploring innate immunity and antiviral defence, where IFIT1 serves as a biomarker of interferon activity.

Performance specifications include a sensitivity of 0.078ng/mL and a detection range of 0.156-10ng/mL. Precision is reported with an intra-assay CV of 7.2% and an inter-assay CV of 11.6%. Linearity has been demonstrated across serial dilutions of serum (95-119%), EDTA plasma (85-105%), and heparin plasma (83-114%) samples. Recovery ranges from 97-109% in serum (average 103%) and 99-111% in plasma (average 105%).

The kit is specific for natural and recombinant human IFIT1 (UniProt P09914), an interferon-induced antiviral RNA-binding protein located in the cytoplasm that acts as a sensor of viral single-stranded RNAs. Suitable sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. For research use only.