Human Inter-alpha-trypsin inhibitor heavy chain H5-like protein (ITIH5L) ELISA Kit

From Assay Genie, this solid-phase sandwich ELISA kit enables quantitative measurement of human Inter-alpha-trypsin inhibitor heavy chain H5-like protein (ITIH5L). The assay is reactive with human samples and validated for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Detection is colorimetric and the kit is intended for research use only.

Performance characteristics supplied by the manufacturer include a detection range of 0.156-10ng/mL and a sensitivity of 0.072ng/mL. Precision is reported as 4.8% intra-assay CV and 8.7% inter-assay CV. Recovery averages 91% for serum (range 85-97%) and 93% for plasma (range 87-99%). Linearity was assessed at 1:2, 1:4, 1:8, and 1:16 dilutions in serum, EDTA plasma, and heparin plasma. The target protein maps to UniProt accession Q6UXX5, with a molecular weight of 143,187 Da, and the antibodies recognise natural and recombinant human Inter-alpha-trypsin inhibitor heavy chain H6.

According to the manufacturer's description, ITIH5L is implicated in inflammation, immune response, and tissue repair, with dysregulation linked to inflammatory conditions, autoimmune disorders, and cancer, supporting its relevance as a potential biomarker. Individual kit components are stored at either -20°C or 4°C, and the product is shipped at +4°C.