Human Integrin Beta 6 (ITGb6) ELISA Kit

The Assay Genie Human Integrin Beta 6 (ITGb6) ELISA Kit enables quantitative measurement of ITGb6 protein in human-derived samples using a colorimetric endpoint read on a microplate. Integrin β6 pairs with the αv subunit to form the αvβ6 heterodimer, a cell-surface receptor that recognizes the RGD motif in extracellular matrix ligands such as fibronectin and the latency-associated peptide of TGF-β. Because αvβ6-mediated activation of latent TGF-β drives epithelial-mesenchymal transition and tissue remodeling, researchers apply this kit to fibrotic disease models, tumor invasion studies, wound-healing research, and biomarker screening in epithelial cancers.

Samples compatible with the workflow include tissue homogenates and cell lysates, supporting both cultured cell experiments and dissected tissue specimens. Each kit provides reagents sufficient for 96 individual assays in a microplate layout, with all buffers, standards, and detection antibody prepared for direct use. The procedure fits standard ELISA workflows and is intended for research use only, allowing laboratories to generate reproducible standard curves for normalizing ITGb6 expression across disease and control cohorts.

Investigators studying head-and-neck, colon, pancreatic, and breast carcinoma often use β6 integrin levels as an indicator of invasive phenotype, while pulmonary and hepatic fibrosis projects track its upregulation in injured epithelium. Assay Genie supplies this kit alongside technical documentation that lists expected optical density ranges, recommended dilutions, and troubleshooting guidance, helping new users establish assay performance in their specific sample matrices.