Human Integrin alpha-M (ITGAM) ELISA Kit

Assay Genie provides a sandwich-format ELISA for the quantitative measurement of human Integrin alpha-M (ITGAM). The colorimetric assay recognises natural and recombinant human Integrin alpha-M, with reactivity for human samples. Compatible sample types include serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. Synonyms listed for the target include CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, CD11B, and CR3A. The reported detection range is 0.312-20ng/mL, with a sensitivity of 0.1ng/mL. Reported precision values are an intra-assay CV of 5.1% and an inter-assay CV of 10.2%.

Integrin alpha-M (UniProt P11215) is described as a single-pass type I membrane protein that forms a heterodimer with a beta-2 subunit. It is implicated in adhesive interactions of monocytes, macrophages, and granulocytes, mediates uptake of complement-coated particles, and acts as a receptor for fibrinogen, factor X, and ICAM1. The product is annotated for the Cancer research area and is labelled for research use only. Kit components have varying storage requirements, with certain elements held at -20°C and others at 4°C.

Reported recovery values are 101% on average (range 95-107) for serum and 103% on average (range 97-109) for plasma. Linearity has been assessed in serum, EDTA plasma, and heparin plasma across dilution factors of 1:2, 1:4, 1:8, and 1:16.