Human Insulin PharmaGenie ELISA Kit (SBRS0795)

Quantitative insulin measurement underpins research in diabetes pathophysiology, β-cell biology, and metabolic disease drug development. The Assay Genie Human Insulin PharmaGenie ELISA Kit supports these workflows with a solid-phase sandwich configuration that pairs pre-coated capture antibodies with a detection antibody for reproducible readouts.

Typical applications include quantification of insulin in cell culture supernatants from pancreatic β-cell lines or isolated islets, alongside blood-derived specimens collected during glucose tolerance testing, insulin resistance studies, and pharmacodynamic monitoring. The chromogenic readout integrates with standard microplate readers, and the kit provides reagents sufficient for 96 individual determinations.

Each package contains a pre-coated microplate, calibration standards, detection antibody, wash buffer, substrate solution, and stop reagent. Standard incubation, washing, and signal development steps are compatible with most automated plate processors. Storage and handling should follow the certificate of analysis, and samples should be aliquoted to limit freeze-thaw cycles.

For research use only, the assay serves academic, pharmaceutical, and biotech laboratories investigating endocrine function, obesity, type 1 and type 2 diabetes mechanisms, and β-cell regeneration strategies.