Human Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) ELISA Kit

Assay Genie offers this Sandwich ELISA Kit for the quantitative measurement of human IGFALS (Insulin-Like Growth Factor Binding Protein, Acid-Labile Subunit), also known by the synonym ALS. The kit reacts with human samples and is validated for use with serum, plasma, tissue homogenates, and other biological fluids, including cell culture supernatates, and is positioned as a sensitive and specific tool for a range of research applications.

The assay operates across a working range of 1.56–100 ng/mL with a stated sensitivity of 0.65 ng/mL. Reported intra-assay precision is CV <10% and inter-assay precision is CV <12%. Spike-and-recovery testing returned average recoveries of 91% in serum, 90% in EDTA plasma, and 84% in heparin plasma, with linearity demonstrated across serial dilutions in the same matrices. The manufacturer states a loss of activity of less than 5% within the expiration date under appropriate storage, giving a shelf life of 12 months. The plate is read at 450 nm following the standard sandwich protocol.

IGFALS is a key regulator of insulin-like growth factor (IGF) signalling and plays a central role in growth and metabolism. Dysregulation of IGFALS has been linked to growth disorders, diabetes, and cancer, making it a useful biomarker for studies in these areas. The kit is labelled for research use only.