Human Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) ELISA Kit

Beyond the capture antibody pre-coated plate provided by Assay Genie, this kit ships with a sealed foil pouch containing desiccant strips to protect unused wells during refrigerated storage. Each batch is supplied with a lot-specific standard curve certificate and the assay procedure accommodates both single-well and duplicate-well standard formats. Researchers investigating tumor biology frequently employ this ELISA as an orthogonal complement to IMP3 immunohistochemistry when quantifying protein abundance across patient-matched tumor and adjacent normal tissue homogenates, particularly in hepatocellular carcinoma and cholangiocarcinoma cohorts where elevated IGF2BP3 correlates with metastatic recurrence. The capture antibody pair has been screened against related IGF2BP family paralogues and shows minimal cross-reactivity, supporting specific quantification of the 64 kDa IGF2BP3 isoform. Recommended sample volume is 50 uL per well, with an assay run time of approximately 3.5 hours excluding overnight incubation steps. Unopened kits remain stable for six months when stored refrigerated, and reconstituted standards should be aliquoted to limit freeze-thaw cycles beyond three rounds.