Human Insulin Like Growth Factor 1 (IGF1) ELISA Kit

Quantitative determination of Insulin-like Growth Factor 1 (IGF-1), a 70-amino acid polypeptide hormone central to the growth hormone axis, in human specimens using a microplate-based antibody-pair immunoassay from Assay Genie. The kit supports research into childhood and adolescent growth disorders, age-related decline in IGF-1, metabolic dysfunction, exercise physiology, and biomarker discovery in translational endocrinology.

Each kit supplies complete reagents for 96 individual determinations: pre-coated microplate wells, recombinant IGF-1 reference standards for building a standard curve, enzyme-labelled detection antibody, wash buffer concentrate, enzymatic substrate solution, stop solution, and a printed protocol booklet. Working volumes, dilution factors, and incubation times for individual procedural steps are described in detail inside the booklet.

Specimens such as serum or plasma from human subjects are added in parallel with standards to the coated wells. Following incubations and washes, the bound analyte is revealed with the substrate and absorbance is read on a standard plate reader, allowing IGF-1 concentrations to be interpolated from the standard curve. Store kit components refrigerated upon arrival and equilibrate to ambient temperature prior to each run. Each lot is quality-controlled for specificity, precision, and reproducibility under routine laboratory conditions.