Human Insulin ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of human insulin using a colorimetric, double-antibody detection method. The microplate provided is pre-coated with a capture antibody specific to the target analyte, and standards and samples are added to the wells to allow the analyte to bind.

According to the manufacturer, the assay delivers a sensitivity of 4.688 pg/mL with a working range of 7.813–500 pg/mL (1 ng = 10 uIU). It has been validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples of human origin. Reported recovery ranges from 87–104% in serum (average 97%), 88–103% in EDTA plasma (average 95%), and 86–105% in heparin plasma (average 97%), with linearity of 81–100% across 1:2 to 1:8 dilutions across the matrices tested. After incubation and washing, a biotinylated detection antibody specific to a separate epitope is added, followed by HRP-conjugated Streptavidin and TMB substrate; the colour reaction is stopped with acidic solution and absorbance is read at 450 nm.

Unopened kits should be stored at 2–8°C for 12 months. The product has been referenced in peer-reviewed work including Amy McGuire et al., "Measurement of energy availability in highly trained male endurance athletes and examination of its associations with bone health and endocrine function," published in the European Journal of Nutrition (PubMed ID: 38953963).